Highly efficient Cas9-mediated transcriptional programming.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25730490.
- Also identified by DOI 10.1038/nmeth.3312 and PMC identifier 4393883.
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Abstract
The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. We demonstrate its utility in activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).
Medical subject headings
- Endonucleases
- Genetic Techniques
- Transcriptional Activation