The cell cycle regulator CCDC6 is a key target of RNA-binding protein EWS.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25751255.
- Also identified by DOI 10.1371/journal.pone.0119066 and PMC identifier 4353705.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Genetic translocation of EWSR1 to ETS transcription factor coding region is considered as primary cause for Ewing sarcoma. Previous studies focused on the biology of chimeric transcription factors formed due to this translocation. However, the physiological consequences of heterozygous EWSR1 loss in these tumors have largely remained elusive. Previously, we have identified various mRNAs bound to EWS using PAR-CLIP. In this study, we demonstrate CCDC6, a known cell cycle regulator protein, as a novel target regulated by EWS. siRNA mediated down regulation of EWS caused an elevated apoptosis in cells in a CCDC6-dependant manner. This effect was rescued upon re-expression of CCDC6. This study provides evidence for a novel functional link through which wild-type EWS operates in a target-dependant manner in Ewing sarcoma.
Medical subject headings
- Bone Neoplasms
- Calmodulin-Binding Proteins
- Cytoskeletal Proteins
- RNA-Binding Proteins
- Sarcoma, Ewing