A permutation test for oligoset DNA pooling studies.

Huang, Hsiao-Yuan; Lin, Jui-Hsiang; Lee, Wen-Chung · PLoS One · 2015

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Abstract

Case-control association studies often suffer from population stratification bias. A previous triple combination strategy of stratum matching, genomic controlling, and multiple DNA pooling can correct the bias and save genotyping cost. However the method requires researchers to prepare a multitude of DNA pools-more than 30 case-control pooling sets in total (polyset). In this paper, the authors propose a permutation test for oligoset DNA pooling studies. Monte-Carlo simulations show that the proposed test has a type I error rate under control and a power comparable to that of individual genotyping. For a researcher on a tight budget, oligoset DNA pooling is a viable option.

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