Proteo-lipobeads for the oriented encapsulation of membrane proteins.

Frank, Pinar; Siebenhofer, Bernhard; Hanzer, Theresa; Geiss, Andreas F; Schadauer, Florian; Reiner-Rozman, Ciril; Durham, Bill; Loew, Leslie M et al. · Soft Matter · 2015

basic_science · Level V

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Abstract

As a surrogate of live cells, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on live cells, for example using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.

Medical subject headings