A plug-and-play ratiometric pH-sensing nanoprobe for high-throughput investigation of endosomal escape.

Deng, Zhou J; Morton, Stephen W; Bonner, Daniel K; Gu, Li; Ow, Hooisweng; Hammond, Paula T · Biomaterials · 2015

basic_science · Level V

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Abstract

An important aspect in the design of nanomaterials for delivery is an understanding of its uptake and ultimate release to the cytosol of target cells. Real-time chemical sensing using a nanoparticle-based platform affords exquisite insight into the trafficking of materials and their cargo into cells. This versatile and tunable technology provides a powerful tool to probe the mechanism of cellular entry and cytosolic delivery of a variety of materials, allowing for a simple and convenient means to screen materials towards efficient delivery of therapeutics such as nucleic acids.

Medical subject headings