Direct visualization of newly synthesized target proteins in situ.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25775042.
- Also identified by DOI 10.1038/nmeth.3319 and PMC identifier 4414919.
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Abstract
Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique that couples noncanonical amino acid tagging or puromycylation with the proximity ligation assay to visualize specific newly synthesized proteins and monitor their origin, redistribution and turnover in situ.
Medical subject headings
- Fibroblasts
- Proteins