Functional annotation of native enhancers with a Cas9-histone demethylase fusion.
basic_science · Level V
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- Record sourced from PubMed, PMID 25775043.
- Also identified by DOI 10.1038/nmeth.3325 and PMC identifier 4414811.
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Abstract
Understanding of mammalian enhancers is limited by the lack of a technology to rapidly and thoroughly test the cell type-specific function. Here, we use a nuclease-deficient Cas9 (dCas9)-histone demethylase fusion to functionally characterize previously described and new enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSD1 at enhancers from previous dCas9-effectors.
Medical subject headings
- Caspase 9
- Enhancer Elements, Genetic
- Histone Demethylases