Small toxic protein encoded on chromosome VII of Saccharomyces cerevisiae.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25781884.
- Also identified by DOI 10.1371/journal.pone.0120678 and PMC identifier 4363601.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
In a previous study, we found an unknown element that caused growth inhibition after its copy number increased in the 3' region of DIE2 in Saccharomyces cerevisiae. In this study, we further identified this element and observed that overexpression of a small protein (sORF2) of 57 amino acids encoded in this region caused growth inhibition. The transcriptional response and multicopy suppression of the growth inhibition caused by sORF2 overexpression suggest that sORF2 overexpression inhibits the ergosterol biosynthetic pathway. sORF2 was not required in the normal growth of S. cerevisiae, and not conserved in related yeast species including S. paradoxus. Thus, sORF2 (designated as OTO1) is an orphan ORF that determines the specificity of this species.
Medical subject headings
- Chromosomes, Fungal
- Open Reading Frames
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins