Enterohemorrhagic Escherichia coli senses low biotin status in the large intestine for colonization and infection.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25791315.
- Also identified by DOI 10.1038/ncomms7592 and PMC identifier 4382993.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Enterohemorrhagic Escherichia coli (EHEC) is an important foodborne pathogen that infects humans by colonizing the large intestine. Here we identify a virulence-regulating pathway in which the biotin protein ligase BirA signals to the global regulator Fur, which in turn activates LEE (locus of enterocyte effacement) genes to promote EHEC adherence in the low-biotin large intestine. LEE genes are repressed in the high-biotin small intestine, thus preventing adherence and ensuring selective colonization of the large intestine. The presence of this pathway in all nine EHEC serotypes tested indicates that it is an important evolutionary strategy for EHEC. The pathway is incomplete in closely related small-intestinal enteropathogenic E. coli due to the lack of the Fur response to BirA. Mice fed with a biotin-rich diet show significantly reduced EHEC adherence, indicating that biotin might be useful to prevent EHEC infection in humans.
Medical subject headings
- Biotin
- Carbon-Nitrogen Ligases
- Escherichia coli Infections
- Escherichia coli O157
- Escherichia coli Proteins
- Intestine, Large
- Intestine, Small
- Repressor Proteins