Single cell transcriptome amplification with MALBAC.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25822772.
- Also identified by DOI 10.1371/journal.pone.0120889 and PMC identifier 4378937.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Recently, Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) has been developed for whole genome amplification of an individual cell, relying on quasilinear instead of exponential amplification to achieve high coverage. Here we adapt MALBAC for single-cell transcriptome amplification, which gives consistently high detection efficiency, accuracy and reproducibility. With this newly developed technique, we successfully amplified and sequenced single cells from 3 germ layers from mouse embryos in the early gastrulation stage, and examined the epithelial-mesenchymal transition (EMT) program among cells in the mesoderm layer on a single-cell level.
Medical subject headings
- Nucleic Acid Amplification Techniques
- Transcriptome