High-speed label-free functional photoacoustic microscopy of mouse brain in action.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25822799.
- Also identified by DOI 10.1038/nmeth.3336 and PMC identifier 4428901.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.
Medical subject headings
- Brain
- Microscopy
- Photoacoustic Techniques