CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25824291.
- Also identified by DOI 10.7554/eLife.05808 and PMC identifier 4379494.
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Abstract
The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.
Medical subject headings
- Calcium
- Fluorescent Dyes
- Indicators and Reagents
- Neurons