Sequencing small genomic targets with high efficiency and extreme accuracy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25849638.
- Also identified by DOI 10.1038/nmeth.3351 and PMC identifier 4414912.
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Abstract
The detection of minority variants in mixed samples requires methods for enrichment and accurate sequencing of small genomic intervals. We describe an efficient approach based on sequential rounds of hybridization with biotinylated oligonucleotides that enables more than 1-million-fold enrichment of genomic regions of interest. In conjunction with error-correcting double-stranded molecular tags, our approach enables the quantification of mutations in individual DNA molecules.
Medical subject headings
- DNA
- Genetic Loci
- Genomics
- Sequence Analysis, DNA