Ex vivo cytosolic delivery of functional macromolecules to immune cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25875117.
- Also identified by DOI 10.1371/journal.pone.0118803 and PMC identifier 4395260.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.
Medical subject headings
- Antibodies
- Dextrans
- Drug Delivery Systems
- Lab-On-A-Chip Devices
- RNA, Small Interfering