Single-Particle Cryo-EM at Crystallographic Resolution.
review · Level V
Where this comes from
- Record sourced from PubMed, PMID 25910205.
- Also identified by DOI 10.1016/j.cell.2015.03.049 and PMC identifier 4409662.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Until only a few years ago, single-particle electron cryo-microscopy (cryo-EM) was usually not the first choice for many structural biologists due to its limited resolution in the range of nanometer to subnanometer. Now, this method rivals X-ray crystallography in terms of resolution and can be used to determine atomic structures of macromolecules that are either refractory to crystallization or difficult to crystallize in specific functional states. In this review, I discuss the recent breakthroughs in both hardware and software that transformed cryo-microscopy, enabling understanding of complex biomolecules and their functions at atomic level.
Medical subject headings
- Crystallography, X-Ray
- Molecular Conformation