G&T-seq: parallel sequencing of single-cell genomes and transcriptomes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25915121.
- Also identified by DOI 10.1038/nmeth.3370.
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Abstract
The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.
Medical subject headings
- DNA
- Genomics
- Nucleic Acid Amplification Techniques
- RNA, Messenger