Organelle-Specific Nitric Oxide Detection in Living Cells via HaloTag Protein Labeling.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25923693.
- Also identified by DOI 10.1371/journal.pone.0123986 and PMC identifier 4414533.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Nitric oxide (NO) is a membrane-permeable signaling molecule that is constantly produced, transferred, and consumed in vivo. NO participates and plays important roles in multiple biological processes. However, spatiotemporal imaging of NO in living cells is challenging. To fill the gap in currently used techniques, we exploited the versatility of HaloTag technology and synthesized a novel organelle-targetable fluorescent probe called HTDAF-2DA. We demonstrate the utility of the probe by monitoring subcellular NO dynamics. The developed strategy enables precise determination of local NO function.
Medical subject headings
- Fluorescent Dyes
- Nitric Oxide
- Organelles