Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25939363.
- Also identified by DOI 10.1021/acs.nanolett.5b00693 and PMC identifier 13093486.
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Abstract
We resolved the organization of subunits in cytoskeletal polymers in cells by light microscopy. Septin GTPases form linear complexes of about 32 nm length that polymerize into filaments. We visualized both termini of septin complexes by single molecule microscopy in vitro. Complexes appeared as 32 nm spaced localization pairs, and filaments appeared as stretches of equidistant localizations. Cellular septins were resolved as localization pairs and thin stretches of equidistant localizations.
Medical subject headings
- Cytoskeleton
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Septins