Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25939423.
- Also identified by DOI 10.1021/acs.nanolett.5b01396 and PMC identifier 4462996.
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Abstract
We employ a novel framework for information-optimal microscopy to design a family of point spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of nanoscale emitters in three dimensions over customizable axial (z) ranges of up to 20 μm with a high numerical aperture objective lens. To illustrate, we perform flow profiling in a microfluidic channel and show scan-free tracking of single quantum-dot-labeled phospholipid molecules on the surface of living, thick mammalian cells.
Medical subject headings
- Cell Membrane
- Lab-On-A-Chip Devices
- Membrane Lipids
- Microscopy
- Quantum Dots