Structural analysis of multicellular organisms with cryo-electron tomography.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25961413.
- Also identified by DOI 10.1038/nmeth.3401.
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Abstract
We developed a method for visualizing tissues from multicellular organisms using cryo-electron tomography. Our protocol involves vitrifying samples with high-pressure freezing, thinning them with cryo-FIB-SEM (focused-ion-beam scanning electron microscopy) and applying fiducial gold markers under cryogenic conditions to the lamellae post-milling. We applied this protocol to acquire tomograms of vitrified Caenorhabditis elegans embryos and worms, which showed the intracellular organization of selected tissues at particular developmental stages in otherwise intact specimens.
Medical subject headings
- Cryoelectron Microscopy
- Electron Microscope Tomography