In vivo single-cell labeling by confined primed conversion.
basic_science · Level V
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- Record sourced from PubMed, PMID 25984699.
- Also identified by DOI 10.1038/nmeth.3405.
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Abstract
Spatially confined green-to-red photoconversion of fluorescent proteins with high-power, pulsed laser illumination is negligible, thus precluding optical selection of single cells in vivo. We report primed conversion, in which low-power, dual-wavelength, continuous-wave illumination results in pronounced photoconversion. With a straightforward addition to a conventional confocal microscope, we show confined primed conversion in living zebrafish and reveal the complex anatomy of individual neurons packed between neighboring cells.
Medical subject headings
- Microscopy, Fluorescence, Multiphoton
- Neurons