Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25996607.
- Also identified by DOI 10.1371/journal.pone.0127802 and PMC identifier 4440651.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We examined the thymoquinone induced inhibition of purified F1 or membrane bound F1FO E. coli ATP synthase. Both purified F1 and membrane bound F1FO were completely inhibited by thymoquinone with no residual ATPase activity. The process of inhibition was fully reversible and identical in both membrane bound F1Fo and purified F1 preparations. Moreover, thymoquinone induced inhibition of ATP synthase expressing wild-type E. coli cell growth and non-inhibition of ATPase gene deleted null control cells demonstrates that ATP synthase is a molecular target for thymoquinone. This also links the beneficial dietary based antimicrobial and anticancer effects of thymoquinone to its inhibitory action on ATP synthase.
Medical subject headings
- ATP Synthetase Complexes
- Benzoquinones
- Enzyme Inhibitors
- Escherichia coli