Detection of Peptide-based nanoparticles in blood plasma by ELISA.
Where this comes from
- Record sourced from PubMed, PMID 25996618.
- Also identified by DOI 10.1371/journal.pone.0126136 and PMC identifier 4440766.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
AIMS: The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. MATERIALS & METHODS: A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. RESULTS: The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl methacrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. CONCLUSIONS: We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
Medical subject headings
- Enzyme-Linked Immunosorbent Assay
- Nanoparticles
- Peptides
- Plasma