Interaction of sulforaphane with DNA and RNA.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26030290.
- Also identified by DOI 10.1371/journal.pone.0127541 and PMC identifier 4452540.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables with anti-inflammatory, anti-oxidant and anti-cancer activities. However, the antioxidant and anticancer mechanism of sulforaphane is not well understood. In the present research, we reported binding modes, binding constants and stability of SFN-DNA and -RNA complexes by Fourier transform infrared (FTIR) and UV-Visible spectroscopic methods. Spectroscopic evidence showed DNA intercalation with some degree of groove binding. SFN binds minor and major grooves of DNA and backbone phosphate (PO2), while RNA binding is through G, U, A bases with some degree of SFN-phosphate (PO2) interaction. Overall binding constants were estimated to be K(SFN-DNA)=3.01 (± 0.035)×10(4) M(-1) and K(SFN-RNA)= 6.63 (±0.042)×10(3) M(-1). At high SFN concentration (SFN/RNA = 1/1), DNA conformation changed from B to A occurred, while RNA remained in A-family structure.
Medical subject headings
- DNA
- Isothiocyanates
- RNA