Functional differentiation of human pluripotent stem cells on a chip.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26030445.
- Also identified by DOI 10.1038/nmeth.3411.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Microengineering human "organs-on-chips" remains an open challenge. Here, we describe a robust microfluidics-based approach for the differentiation of human pluripotent stem cells directly on a chip. Extrinsic signal modulation, achieved through optimal frequency of medium delivery, can be used as a parameter for improved germ layer specification and cell differentiation. Human cardiomyocytes and hepatocytes derived on chips showed functional phenotypes and responses to temporally defined drug treatments.
Medical subject headings
- Microfluidic Analytical Techniques
- Pluripotent Stem Cells