Plasmodium falciparum genetic crosses in a humanized mouse model.

Vaughan, Ashley M; Pinapati, Richard S; Cheeseman, Ian H; Camargo, Nelly; Fishbaugher, Matthew; Checkley, Lisa A; Nair, Shalini; Hutyra, Carolyn A et al. · Nat Methods · 2015

basic_science · Level V

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Abstract

Genetic crosses of phenotypically distinct strains of the human malaria parasite Plasmodium falciparum are a powerful tool for identifying genes controlling drug resistance and other key phenotypes. Previous studies relied on the isolation of recombinant parasites from splenectomized chimpanzees, a research avenue that is no longer available. Here we demonstrate that human-liver chimeric mice support recovery of recombinant progeny for the identification of genetic determinants of parasite traits and adaptations.

Medical subject headings