Application of BRET to monitor ligand binding to GPCRs.
basic_science · Level V
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- Record sourced from PubMed, PMID 26030448.
- Also identified by DOI 10.1038/nmeth.3398 and PMC identifier 4488387.
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Abstract
Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.
Medical subject headings
- Bioluminescence Resonance Energy Transfer Techniques
- Receptors, G-Protein-Coupled