Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26039892.
- Also identified by DOI 10.1002/adhm.201500021 and PMC identifier 4529809.
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Abstract
Monodisperse alginate microgels (10-50 μm) are created via droplet-based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.
Medical subject headings
- Alginates
- Gels
- Microfluidic Analytical Techniques