Effects of Hypergravity on Osteopontin Expression in Osteoblasts.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26046934.
- Also identified by DOI 10.1371/journal.pone.0128846 and PMC identifier 4457898.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Mechanical stimuli play crucial roles in bone remodeling and resorption. Osteopontin (OPN), a marker for osteoblasts, is important in cell communication and matrix mineralization, and is known to function during mechanotransduction. Hypergravity is a convenient approach to forge mechanical stimuli on cells. It has positive effects on certain markers of osteoblast maturation, making it a possible strategy for bone tissue engineering. We investigated the effects of hypergravity on OPN expression and cell signaling in osteoblasts. Hypergravity treatment at 20 g for 24 hours upregulated OPN expression in MC3T3-E1 cells at the protein as well as mRNA level. Hypergravity promoted OPN expression by facilitating focal adhesion assembly, strengthening actin bundles, and increasing Runx2 expression. In the hypergravity-triggered OPN expression pathway, focal adhesion assembly-associated FAK phosphorylation was upstream of actin bundle assembly.
Medical subject headings
- Core Binding Factor Alpha 1 Subunit
- Focal Adhesions
- Hypergravity
- Mechanotransduction, Cellular
- Osteoblasts
- Osteopontin
- RNA, Messenger