A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26055071.
- Also identified by DOI 10.1039/c5lc00443h and PMC identifier 4627644.
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Abstract
We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing.
Medical subject headings
- Bioreactors
- Human Growth Hormone
- Interferon-alpha
- Microfluidic Analytical Techniques
- Perfusion
- Pichia
- Recombinant Proteins