Steady-state cross-correlations for live two-colour super-resolution localization data sets.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26066572.
- Also identified by DOI 10.1038/ncomms8347 and PMC identifier 4467025.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Cross-correlation of super-resolution images gathered from point localizations allows for robust quantification of protein co-distributions in chemically fixed cells. Here this is extended to dynamic systems through an analysis that quantifies the steady-state cross-correlation between spectrally distinguishable probes. This methodology is used to quantify the co-distribution of several mobile membrane proteins in both vesicles and live cells, including Lyn kinase and the B-cell receptor during antigen stimulation.
Medical subject headings
- Color
- Proteins