Correlation Between the Interval of Influenza Virus Infectivity and Results of Diagnostic Assays in a Ferret Model.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26068783.
- Also identified by DOI 10.1093/infdis/jiv331 and PMC identifier 4704662.
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Abstract
The relationship between influenza virus infectivity and virus shedding, based on different diagnostic methods, has not been defined. Three donor ferrets infected with 2009 pandemic influenza A(H1N1) underwent daily quantitative culture, antigen-detection testing, and real-time reverse transcription-polymerase chain reaction (RT-PCR). Eight contacts were sequentially cohoused with each of the donors for 24 hours during days 3-10 after inoculation. Transmission was observed until day 5 after inoculation, corresponding to high culture titers and positive results of antigen-detection tests. Real-time RT-PCR showed no relation to the cessation of transmission. Antigen-detection testing and virus culture but not real-time RT-PCR identified the end of the infectious period.
Medical subject headings
- Influenza A Virus, H1N1 Subtype
- Orthomyxoviridae Infections
- Virus Shedding