Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26073070.
- Also identified by DOI 10.1038/ncomms8276 and PMC identifier 4490402.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Adaptive optics by direct imaging of the wavefront distortions of a laser-induced guide star has long been used in astronomy, and more recently in microscopy to compensate for aberrations in transparent specimens. Here we extend this approach to tissues that strongly scatter visible light by exploiting the reduced scattering of near-infrared guide stars. The method enables in vivo two-photon morphological and functional imaging down to 700 μm inside the mouse brain.
Medical subject headings
- Brain
- Intravital Microscopy
- Light
- Optical Imaging