A complete bacterial genome assembled de novo using only nanopore sequencing data.
basic_science · Level V
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- Record sourced from PubMed, PMID 26076426.
- Also identified by DOI 10.1038/nmeth.3444.
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Abstract
We have assembled de novo the Escherichia coli K-12 MG1655 chromosome in a single 4.6-Mb contig using only nanopore data. Our method has three stages: (i) overlaps are detected between reads and then corrected by a multiple-alignment process; (ii) corrected reads are assembled using the Celera Assembler; and (iii) the assembly is polished using a probabilistic model of the signal-level data. The assembly reconstructs gene order and has 99.5% nucleotide identity.
Medical subject headings
- Computational Biology
- Escherichia coli K12
- Genome, Bacterial
- Nanopores
- Nanotechnology
- Sequence Analysis, DNA