Rh D blood group conversion using transcription activator-like effector nucleases.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26078220.
- Also identified by DOI 10.1038/ncomms8451 and PMC identifier 4490371.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Group O D-negative blood cells are universal donors in transfusion medicine and methods for converting other blood groups into this universal donor group have been researched. However, conversion of D-positive cells into D-negative is yet to be achieved, although conversion of group A or B cells into O cells has been reported. The Rh D blood group is determined by the RHD gene, which encodes a 12-transmembrane domain protein. Here we convert Rh D-positive erythroid progenitor cells into D-negative cells using RHD-targeting transcription activator-like effector nucleases (TALENs). After transfection of TALEN-encoding plasmids, RHD-knockout clones are obtained. Erythroid-lineage cells differentiated from these knockout erythroid progenitor cells do not agglutinate in the presence of anti-D reagents and do not express D antigen, as assessed using flow cytometry. Our programmable nuclease-induced blood group conversion opens new avenues for compatible donor cell generation in transfusion medicine.
Medical subject headings
- Deoxyribonucleases
- Erythrocytes
- Erythroid Precursor Cells
- Gene Knockout Techniques
- Gene Transfer Techniques
- RNA, Messenger
- Rh-Hr Blood-Group System