Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26083372.
- Also identified by DOI 10.1371/journal.pone.0130904 and PMC identifier 4470828.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that "light" plasma membranes have a high carotenoid/protein ratio, when compared to "heavier" plasma membranes or thylakoids. The localization of CrtQ and CrtO, two well-defined carotenoid synthesis pathway enzymes in Synechocystis, was studied by epitope tagging and western blots. Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of β-carotene precursor molecules and echinenone.
Medical subject headings
- Bacterial Proteins
- Carotenoids
- Cell Membrane
- Synechocystis