Isolation of High-Purity Extracellular Vesicles by Extracting Proteins Using Aqueous Two-Phase System.
Where this comes from
- Record sourced from PubMed, PMID 26090684.
- Also identified by DOI 10.1371/journal.pone.0129760 and PMC identifier 4475045.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We present a simple and rapid method to isolate extracellular vesicles (EVs) by using a polyethylene glycol/dextran aqueous two-phase system (ATPS). This system isolated more than ~75% of melanoma-derived EVs from a mixture of EVs and serum proteins. To increase the purity of EVs, a batch procedure was combined as additional steps to remove protein contaminants, and removed more than ~95% of the protein contaminants. We also performed RT-PCR and western blotting to verify the diagnostic applicability of the isolated EVs, and detected mRNA derived from melanoma cells and CD81 in isolated EVs.
Medical subject headings
- Extracellular Vesicles
- Liquid-Liquid Extraction
- Proteins