Single-cell magnetic imaging using a quantum diamond microscope.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26098019.
- Also identified by DOI 10.1038/nmeth.3449 and PMC identifier 4521973.
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Abstract
We apply a quantum diamond microscope for detection and imaging of immunomagnetically labeled cells. This instrument uses nitrogen-vacancy (NV) centers in diamond for correlated magnetic and fluorescence imaging. Our device provides single-cell resolution and a field of view (∼1 mm(2)) two orders of magnitude larger than that of previous NV imaging technologies, enabling practical applications. To illustrate, we quantified cancer biomarkers expressed by rare tumor cells in a large population of healthy cells.
Medical subject headings
- Image Processing, Computer-Assisted
- Magnetic Phenomena
- Microscopy
- Single-Cell Analysis