A naturally monomeric infrared fluorescent protein for protein labeling in vivo.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26098020.
- Also identified by DOI 10.1038/nmeth.3447 and PMC identifier 4521985.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its homologs in protein labeling. Drawing on structural analysis of the dimer interface, we identified a bacteriophytochrome in the sequence database that is monomeric in truncated form and engineered it into a naturally monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live cells, Drosophila and zebrafish. It should be useful in molecular, cell and developmental biology.
Medical subject headings
- Green Fluorescent Proteins
- Infrared Rays