cir-ITCH plays an inhibitory role in colorectal cancer by regulating the Wnt/β-catenin pathway.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26110611.
- Also identified by DOI 10.1371/journal.pone.0131225 and PMC identifier 4482251.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Noncoding RNAs (ncRNAs) are the dominant product of eukaryotic transcription. These products range from short microRNAs (miRNAs) to long intergenic noncoding RNAs (lincRNAs). Circular RNAs composed of exonic sequences represent an understudied form of ncRNA that was discovered more than 20 years ago. Using a TaqMan-based reverse transcriptase polymerase chain reaction assay, we analyzed the relationship between cir-ITCH expression and colorectal cancer (CRC) in a total of 45 CRCs and paired adjacent non-tumor tissue samples. We found that cir-ITCH expression was typically down-regulated in CRC compared to the peritumoral tissue. This result, as well as several follow-up experiments, showed that cir-ITCH could increase the level of ITCH, which is involved in the inhibition of the Wnt/β-catenin pathway. Therefore, our results showed that cir-ITCH plays a role in CRC by regulating the Wnt/β-catenin pathway.
Medical subject headings
- Colorectal Neoplasms
- Gene Expression Regulation, Neoplastic
- MicroRNAs
- RNA
- RNA, Untranslated
- Wnt Signaling Pathway