Characterization of the Prokaryotic Sodium Channel NavSp Pore with a Microfluidic Bilayer Platform.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26147601.
- Also identified by DOI 10.1371/journal.pone.0131286 and PMC identifier 4493117.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
This paper describes the use of a newly-developed micro-chip bilayer platform to examine the electrophysiological properties of the prokaryotic voltage-gated sodium channel pore (Na(v)Sp) from Silicibacter pomeroyi. The platform allows up to 6 bilayers to be analysed simultaneously. Proteoliposomes were incorporated into suspended lipid bilayers formed within the microfluidic bilayer chips. The chips provide access to bilayers from either side, enabling the fast and controlled titration of compounds. Dose-dependent modulation of the opening probability by the channel blocking drug nifedipine was measured and its IC50 determined.
Medical subject headings
- Ion Channel Gating
- Microfluidics
- Rhodobacteraceae
- Sodium Channels