The Cold Shock Domain of YB-1 Segregates RNA from DNA by Non-Bonded Interactions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26147853.
- Also identified by DOI 10.1371/journal.pone.0130318 and PMC identifier 4493011.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The human YB-1 protein plays multiple cellular roles, of which many are dictated by its binding to RNA and DNA through its Cold Shock Domain (CSD). Using molecular dynamics simulation approaches validated by experimental assays, the YB1 CSD was found to interact with nucleic acids in a sequence-dependent manner and with a higher affinity for RNA than DNA. The binding properties of the YB1 CSD were close to those observed for the related bacterial Cold Shock Proteins (CSP), albeit some differences in sequence specificity. The results provide insights in the molecular mechanisms whereby YB-1 interacts with nucleic acids.
Medical subject headings
- Cold Shock Proteins and Peptides
- DNA
- RNA
- Y-Box-Binding Protein 1