DNA Brushing Shoulders: Targeted Looping and Scanning of Large DNA Strands.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26156085.
- Also identified by DOI 10.1021/acs.nanolett.5b02476 and PMC identifier 4684187.
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Abstract
We present a nanofluidic device for targeted manipulations in the quarternary structure of single DNA molecules. We demonstrate the folding and unfolding of hairpin-shaped regions, similar to chromatin loops. These loops are stable for minutes at nanochannel junctions. We demonstrate continuous scanning of two DNA segments that occupy a common nanovolume. We present a model governing the stability of loop folds and discuss how the system achieves specific DNA configurations without operator intervention.
Medical subject headings
- DNA