MicroRNA-183 increases osteoclastogenesis by repressing heme oxygenase-1.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26163109.
- Also identified by DOI 10.1016/j.bone.2015.07.006.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Emerging evidence suggests that microRNAs (miRs) influence skeletal structure by modulating osteoclastogenesis and bone resorption. We have demonstrated previously that the up-regulation of heme oxygenase-1 (HO-1) attenuated osteoclastogenesis in bone marrow-derived macrophages (BMMs). RANKL-induced osteoclastogenesis elevates microRNA-183 (miR-183) in BMM. We show here that HO-1 is a target gene of miR-183 and that this miRNA binds to the 3'-UTR of HO-1. We find that a synthetic inhibitor that binds to miR-183 decreases osteoclast (OC) differentiation and increases the expression of HO-1, while a mimic of endogenous mature miR-183 has the opposite effect. Moreover, the HO-1 inducers, resveratrol and piceatannol decrease the expression of miR-183, resulting in attenuated osteoclastogenesis. Our findings reveal how miR-183 affects OC formation.
Medical subject headings
- Heme Oxygenase-1
- MicroRNAs
- Osteoclasts
- Osteogenesis