Sirtuin Inhibition Adversely Affects Porcine Oocyte Meiosis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26176547.
- Also identified by DOI 10.1371/journal.pone.0132941 and PMC identifier 4503529.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Sirtuins have been implicated in diverse biological processes, including oxidative stress, energy metabolism, cell migration, and aging. Here, we employed Sirtuin inhibitors, nicotinamide (NAM) and Sirtinol, to investigate their effects on porcine oocyte maturation respectively. The rate of polar body extrusion in porcine oocytes decreased after treatment with NAM and Sirtinol, accompanied with the failure of cumulus cell expansion. We further found that NAM and Sirtinol significantly disrupted oocyte polarity, and inhibited the formation of actin cap and cortical granule-free domain (CGFD). Moreover, the abnormal spindles and misaligned chromosomes were readily detected during porcine oocyte maturation after treatment with NAM and Sirtinol. Together, these results suggest that Sirtuins are involved in cortical polarity and spindle organization in porcine oocytes.
Medical subject headings
- Benzamides
- Histone Deacetylase Inhibitors
- Meiosis
- Naphthols
- Niacinamide
- Oocytes
- Sirtuins