High-Throughput Method of Whole-Brain Sectioning, Using the Tape-Transfer Technique.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26181725.
- Also identified by DOI 10.1371/journal.pone.0102363 and PMC identifier 4504703.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Cryostat sectioning is a popular but labor-intensive method for preparing histological brain sections. We have developed a modification of the commercially available CryoJane tape collection method that significantly improves the ease of collection and the final quality of the tissue sections. The key modification involves an array of UVLEDs to achieve uniform polymerization of the glass slide and robust adhesion between the section and slide. This report presents system components and detailed procedural steps, and provides examples of end results; that is, 20 μm mouse brain sections that have been successfully processed for routine Nissl, myelin staining, DAB histochemistry, and fluorescence. The method is also suitable for larger brains, such as rat and monkey.
Medical subject headings
- Brain
- Cryoultramicrotomy
- Microtomy
- Staining and Labeling