Inferring transient particle transport dynamics in live cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 26192083.
- Also identified by DOI 10.1038/nmeth.3483 and PMC identifier 4733533.
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Abstract
Live-cell imaging and particle tracking provide rich information on mechanisms of intracellular transport. However, trajectory analysis procedures to infer complex transport dynamics involving stochastic switching between active transport and diffusive motion are lacking. We applied Bayesian model selection to hidden Markov modeling to infer transient transport states from trajectories of mRNA-protein complexes in live mouse hippocampal neurons and metaphase kinetochores in dividing human cells. The software is available at http://hmm-bayes.org/.
Medical subject headings
- Actins
- Hippocampus
- Models, Biological
- Molecular Imaging
- Neurons