Engineered CHO cells for production of diverse, homogeneous glycoproteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26192319.
- Also identified by DOI 10.1038/nbt.3280.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.
Medical subject headings
- Glycoproteins
- Protein Engineering
- Recombinant Proteins