Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26203778.
- Also identified by DOI 10.1021/acs.nanolett.5b01047 and PMC identifier 4675668.
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Abstract
We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.
Medical subject headings
- Fluorescence Resonance Energy Transfer
- Fluorescent Dyes
- Matrix Metalloproteinase 14
- Neoplasms
- Peptides
- Single-Cell Analysis