Peptide Bioink: Self-Assembling Nanofibrous Scaffolds for Three-Dimensional Organotypic Cultures.

Loo, Yihua; Lakshmanan, Anupama; Ni, Ming; Toh, Lai Ling; Wang, Shi; Hauser, Charlotte A E · Nano Lett · 2015

basic_science · Level V

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Abstract

Printable scaffolds with adequate mechanical strength and stiffness are sought after to ensure viability of printed cells and tissues. We report the first peptide bioinks-lysine-containing hexapeptides that self-assemble into stable, nanofibrous three-dimensional hydrogels with unprecedented stiffness of up to 40 kPa. These biocompatible scaffolds support the three-dimensional culture of human stem cells and differentiation of primary cells into organotypic (gastrointestinal and skin) structures for high-throughput screening, diagnosis, and tissue engineering.

Medical subject headings